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3DJH RI
3.0] Materials and Method:
3.1) Solvents:
Solvent Name
Grade
Product No.
Batch No.
Supplier
Acetonitrile
HPLC
A0755
R014F06
Rankem
Methanol
HPLC
M0275
R246A07
R189A07
R180M06
Rankem
Water
Ultra pure Grade
From Sartorius water purification system.
3.2) Chemicals and their details
3.2.1)
Reference Standard:
Withanoside IV, Withanoside V, Withaferin A, 12-Deoxy Withastramonolide,
Withanolide A, Withanolide B Reference standard with % purity >90 %.
Reference standard
Lot No.
% Purity
Supplier
Withanoside IV
T6B107
90% w/w
Withanoside V
T5A096
90% w/w
Withaferin A
T7A047
99% w/w
12- Deoxy withastramonolide
T7A099
95% w/w
Withanolide A
T6K051
99% w/w
Withanolide B
T6A088
99% w/w
Natural Remedies
Private Limited,
Bangalore
3.2.2) Other sources of reference standard: M/s. Chromadex and M/s. Extrasynthese
3.2.3) Sample and their details:
Sample
Standardized to
Batch No
Raw material
Total withanolides >0.2% by HPLC
RD/1162
Raw material
Total withanolides >0.2% by HPLC
ERH-046
Water extract
Total withanolides >0.15% by HPLC
WS–05Lot20
Hydro-alcoholic extract
Total withanolides >1.0% by HPLC
WS–05Lot21
Methanolic extract
Total withanolides >1.5% by HPLC
RD–1045
Methanolic extract
Total withanolides >2.5% by HPLC
WS–06Lot08
Methanolic extract
Total withanolides >2.5% by HPLC
WS–06Lot10
Methanolic extract
Total withanolides >2.5% by HPLC
RD–1170
QC Check sample:
The QC check sample is the reference extract of
Withania somnifera
extract.
It is prepared out of botanically authenticated
Withania somnifera
and confirmed for the presence
of Withanoside IV, Withanoside V, Withaferin A, 12-Deoxy withastramonolide, Withanolide A and
Withanolide B using the respective reference standards.