169 / 227
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3DJH RI
Possible matrix:
Other secondary metabolites like alkaloids, phytosterols, proteins, carbohydrate,
and excipients like Colloidal silicon dioxide, Light magnesium carbonate or any other inorganic
salts
.
3.3) Equipments:
3.3.1) Analytical Weighing Balance:
Balance Name
Instrument
Code
Description
Sartorius-BP-211D
QCD/CIF/EB/01
Weighing capacity 1mg to 210gm (max)
with 0.01mg accuracy
Sartorius-CP-245D
QCD/AHP/EB/01 Weighing capacity 1mg to 220gm (max)
with 0.1mg accuracy
3.3.2) HPLC:
System
Instrumental
code
Description
HPLC
QCD/CIF/HPLC/03
SHIMADZU LC 2010 A with system controller unit,
degasser unit, low pressure gradient unit up to 4 solvents,
pump unit with seal unit mechanism, mixer, auto sampler,
column oven, UV-VIS detector, LC solution
HPLC
QCD/CIF/HPLC/02
Shimadzu LC10A system equipped with binary
LC10ADVP pump, with SIL10AD
vp
auto sampler,
SPD-M 10A
vp
Photo Diode Array Detector, SCL 10AVP
system controller, CTO 10AVP column oven in combination
with Class VP software version 6.03
3.3.3) Column:
Column
Column code
Column
No.
Description
ODS
C
18
Silica
QC-18-59
625381
Merck kGaA, Hibar ® RT250-4.9; Prepacked
Column; Cat1.01886;
Lichrospher® 100;
RP-18(5µm);
Lot L56139833; No. 625381
C
18
QC-18-57
359208-12
Phenomenex, Luna
5 µ C
18
(2)100 A,
250 X 4.60 mm, 5 microns
Part No. :00G-4252- E0
No. 359208-12
C
18
QC-18-61
S.N.0170100159
Grace Smart RP 18 5µ
Lot No.39/085
Col. No. S.N.0170100159 PN 5138810
3.3.4) Sonicator:
System
Instrument Code
Description
SIDILU UTRASONICS
SONICATOR
QCD/AHP/SON/01
50 frequency/sec,
Ultrasonic frequency 37±3,230 Volt,
adjust for 1-9 min
BANDELIN SONOREX
QCD/CIF/SON/01
230Volt
§
+]
HF-frequency 35KHz,adjust for
1-15 min