175 / 227
1$785$/ 5(0(',(6
3DJH RI
Table-3: Data from sample chromatogram
UV detector
PDA detector
Substance
Compound
Retention time
(minutes)
Relative
retention
time
Retention
time
(minutes)
Relative
retention time
Withanoside IV
15.516
0.698
16.373
0.705
Withanoside V
19.692
0.885
20.565
0.886
Withaferin A
20.366
0.916
21.376
0.921
12-Deoxy
withastramonolide
21.379
0.961
22.368
0.964
Withanolide A
22.242
1.0
23.211
1.0
Standard mix
Withanolide B
25.539
1.148
26.475
1.141
Withanoside IV
15.376
0.695
16.576
0.713
Withanoside V
19.537
0.883
20.672
0.889
Withaferin A
20.226
0.914
21.440
0.922
12-Deoxy
withastramonolide
21.263
0.961
22.400
0.964
Withanolide A
22.128
1.0
23.243
1.0
Extract
Withanolide B
25.447
1.149
26.496
1.140
Withanoside IV
15.547
0.699
16.576
0.713
Withanoside V
19.698
0.886
20.661
0.889
Withaferin A
20.372
0.916
21.440
0.922
12-Deoxy
withastramonolide
21.377
0.961
22.389
0.963
Withanolide A
22.239
1.0
23.243
1.0
Raw material
Withanolide B
25.523
1.148
26.496
1.140