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4.2) Linearity:
4.2.1) Definition
: The linearity is ability of an analytical procedure to elicit response linearity
related to the amount or concentration within the specified range.
4.2.2) Assessment:
By plotting a graph using concentrations on X-axis and the respective peak
areas on Y-axis. Correlation co-efficient
r
2
is calculated.
4.2.3) Acceptance Criterion:
co-efficient
r
2
should be > 0.98.
4.2.4) Experiment:
a)
Standard Preparation:
6.60 mg of Withanoside IV, 6.0 mg of Withanoside V, 7.38 mg of
Withaferin A, 7.11 mg of 12-Deoxy withastramonolide, 6.91 mg of Withanolide A and 6.75
mg of Withanolide B were dissolved in 10ml methanol by gently heating, cooled and made
up to 50ml with methanol.
Stock solution.
The concentration of each analyte in 5 ml is;
Withanoside IV = 7.34 x 90= 6.606 mg
100
Withanoside V = 6.67 x 90 = 6.003 mg
100
Withaferin A = 7.45x 99 = 7.3755 mg
100
12-Deoxy withastramonolide = 7.48x 95 = 7.106 mg
100
Withanolide A=6.98x 99 = 6.9102 mg
100
Withanolide B= 6.82x 99 = 6.7518 mg
100