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3DJH RI
Table-4: Stock solution
Sl. No.
Reference standard
Concentrations (mcg/ml)
1.
Withanoside IV
1321.20
2.
Withanoside V
1200.60
3.
Withaferin A
1475.10
4.
12-Deoxy withastramonolide
1421.20
5.
Withanolide A
1382.04
6.
Withanolide B
1350.36
Dilution-1:
5ml of stock solution was pipetted to10ml volumetric flask and made up to 10ml with
acetonitrile.
Table-5
Sl. No.
Reference standard
Concentrations (mcg/ml)
1.
Withanoside IV
660.60
2.
Withanoside V
600.30
3.
Withaferin A
737.55
4.
12-Deoxy withastramonolide
710.60
5.
Withanolide A
691.02
6.
Withanolide B
675.18
Dilution-2:
5ml of Dilution-1 solution was pipetted to10ml volumetric flask and made up to 10ml
with acetonitrile.
Table-6
Sl. No.
Reference standard
Concentrations (mcg/ml)
7.
Withanoside IV
330.30
8.
Withanoside V
300.15
9.
Withaferin A
368.78
10.
12-Deoxy withastramonolide
355.30
11.
Withanolide A
345.51
12.
Withanolide B
337.59