183 / 227
1$785$/ 5(0(',(6
3DJH RI
Dilution-3:
5ml of Dilution-2 solution was pipetted to10ml volumetric flask and made up to 10ml
with acetonitrile.
Table-7
Sl. No.
Reference standard
Concentrations (mcg/ml)
1.
Withanoside IV
165.15
2.
Withanoside V
150.08
3.
Withaferin A
184.39
4.
12-Deoxy withastramonolide
177.65
5.
Withanolide A
172.76
6.
Withanolide B
168.79
Dilution-4:
5ml of Dilution-3 solution was pipetted to10ml volumetric flask and made up to 10ml
with acetonitrile.
Table-8
Sl. No.
Reference standard
Concentrations (mcg/ml)
1.
Withanoside IV
82.58
2.
Withanoside V
75.04
3.
Withaferin A
92.19
4.
12-Deoxy withastramonolide
88.83
5.
Withanolide A
86.38
6.
Withanolide B
84.39
Dilution-5:
5ml of Dilution-4 solution was pipetted to10ml volumetric flask and made up to 10ml
with acetonitrile.
Table-9
Sl. No.
Reference standard
Concentrations (mcg/ml)
1.
Withanoside IV
41.29
2.
Withanoside V
37.52
3.
Withaferin A
46.09
4.
12-Deoxy withastramonolide
44.41
5.
Withanolide A
43.19
6.
Withanolide B
42.19