189 / 227
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3DJH RI
PEAK AREA OF WITHANOLIDE A
Table-15
Different Concentrations
Withanolide A
(mcg/ml)
Dilution -6
(21.59)
Dilution -5
(43.19)
Dilution -4
(86.38)
Dilution -3
(172.76)
Dilution -2
(345.51)
Dilution -1
(691.02)
S. solution
(1382.04)
Replicate – 1
551317
1084798
2130939
4137256
7955602
13463661
13878628
Replicate –2
550721
1084624
2130222
4140860
7957220
13489137
13809664
Replicate –3
550930
1084955
2130126
4118314
7958121
13484780
13766317
Replicate –4
550882
1084918
2112327
4144205
7963123
13502916
13721170
Replicate – 5
552430
1082926
2131439
4149692
7973789
13491381
13664575
Mean
551256
1084444
2127011
4138065
7961571
13486375
13768071
Response factor
Area /
concentration
25527.76
25109.41
24624.59
23953.38
23042.95
19516.62
9962.14
Mean
(Dilution 3 to 6)
24803.79
RSD
(Dilution 3 to 6)
2.36
LINEARITY GRAPH PEAK AREA Vs CONCENTRATION
0 100 200 300 400 500 600 700 800
0
5000000
10000000
15000000
Best-fit values
Slope
Y-intercept when X=0.0
X-intercept when Y=0.0
1/slope
95% Confidence Intervals
Slope
Y-intercept when X=0.0
X-intercept when Y=0.0
Goodness of Fit
r²
Sy.x
Is slope significantly non-zero?
F
DFn, DFd
P value
Deviation from zero?
Data
Number of X values
Maximum number of Y replicates
Total number of values
Number of missing values
19430 ± 335.7
486700 ± 109300
-25.05
0.00005148
18740 to 20110
262700 to 710600
-45.92 to -4.814
0.9917
430000
3348
1.000, 28.00
< 0.0001
Significant
6
5
30
0
Concentration of Withanolide A (mcg/ml)
Area