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3DJH RI

4.2.6) Conclusion:

Correlation Coefficient (r

2

) for Withanoside IV was found to be 0.9999

Correlation Coefficient (r

2

) for Withanoside V was found to be 0.9999

Correlation Coefficient (r

2

) for Withaferin A was found to be 0.9957

Correlation Coefficient (r

2

) for 12 Deoxy Withastramonolide was found to be 0.9862

Correlation Coefficient (r

2

) for Withanolide A was found to be 0.9917

Correlation Coefficient (r

2

) for Withanolide B was found to be 0.9840

Correlation coefficient (r

2

) found to be >0.99

for both the analytes indicating the linearity and the

method is linear between the below concentrations.

Withanoside IV – 20. 6 and 330.0 mcg

Withanoside V – 18.7 and 300.15 mcg

Withaferin A – 23.0 and 184.0 mcg

12 Deoxy Withastramonolide – 22.0 and 177.0mcg

Withanolide A – 21.0 and 172.0 mcg

Withanolide B – 21.0 and 169.0 mcg

Enclosure-5:

Refer standard chromatograms

(35 Pages)