197 / 227
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3DJH RI
Precision of Retention time:
4.3.10) Observation
Table-30: Retention time of Withanoside IV
Different Concentrations
Withanoside IV
(mcg/ml)
Dilution -6
(20.64)
Dilution -5
(41.29)
Dilution -4
(82.58)
Dilution -3
(165.15)
Dilution -2
(330.30)
Dilution -1
(660.60)
S. solution
(1321.20)
Replicate – 1
15.466
15.499
15.523
15.539
15.489
15.732
15.513
Replicate – 2
15.481
15.479
15.533
15.539
15.5
15.53
15.516
Replicate – 3
15.474
15.511
15.512
15.504
15.485
15.502
15.517
Replicate – 4
15.467
15.494
15.513
15.534
15.497
15.519
15.513
Replicate – 5
15.483
15.509
15.52
15.502
15.68
15.515
15.511
Mean
15.474
15.498
15.520
15.524
15.530
15.559
15.514
RSD
0.05
0.08
0.05
0.12
0.54
0.62
0.02
Table-31: Retention time of Withanoside IV in sample (B. No. WS/06Lot10)
Replicate
Conc-1
(753.6 mg/100ml)
Conc-2
(1503.2 mg/100ml)
Conc-3
(2096.0 mg/100ml)
Replicate - 1
15.529
15.383
15.462
Replicate - 2
15.372
15.388
15.390
Mean
15.451
15.386
15.426
RSD
0.51
0.02
0.23
Table-32: Retention time of Withanoside V
Different Concentrations
Withanoside V
(mcg/ml)
Dilution -6
(18.76)
Dilution -5
(37.52)
Dilution -4
(75.04)
Dilution -3
(150.08)
Dilution -2
(300.15)
Dilution -1
(600.30)
S. solution
(1200.60)
Replicate – 1
19.651
19.657
19.708
19.721
19.676
19.926
19.688
Replicate – 2
19.641
19.664
19.862
19.686
19.689
19.689
19.695
Replicate – 3
19.658
19.67
19.694
19.691
19.648
19.687
19.702
Replicate – 4
19.635
19.668
19.674
19.691
19.697
19.684
19.706
Replicate – 5
19.665
19.664
19.68
19.688
19.877
19.676
19.704
Mean
19.650
19.665
19.724
19.695
19.717
19.732
19.699
RSD
0.06
0.03
0.40
0.07
0.46
0.55
0.04