ASH-001_AOAC_Withania_Submission

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1$785$/ 5(0(',(6 3DJH RI
Table-48: B.
Replicate Withanoside
IV
Withanoside
V
Withaferin
A
12-Deoxy
withastramonolide
Withanolide A Withanolide
B
Sum
Replicate - 1 0.603 0.361 0.399 0.247 0.130 0.042 1.782
Replicate - 2 0.603 0.366 0.400 0.247 0.130 0.042 1.788
Replicate - 3 0.610 0.365 0.407 0.251 0.132 0.043 1.809
Mean 0.606 0.364 0.402 0.248 0.131 0.042 1.793
RSD 0.68 0.79 1.09 0.95 1.09 0.97 0.80
Table-49: B.
Replicate Withanoside
IV
Withanoside
V
Withaferin
A
12-Deoxy
withastramonolide
Withanolide A Withanolide
B
Sum
Replicate - 1 0.150 0.089 0.099 0.021 0.025 0.004 0.388
Replicate - 2 0.153 0.090 0.101 0.021 0.025 0.004 0.395
Replicate - 3 0.152 0.090 0.100 0.020 0.024 0.004 0.390
Mean 0.152 0.090 0.100 0.021 0.025 0.004 0.391
RSD 1.06 0.74 0.75 1.0 1.06 1.24 0.86
Table-50: B. No.ERH/46
Replicate Withanoside
IV
Withanoside
V
Withaferin
A
12-Deoxy
withastramonolide
Withanolide A Withanolide
B
Sum
Replicate - 1 0.185 0.149 0.146 0.041 0.041 0.010 0.573
Replicate - 2 0.182 0.146 0.144 0.041 0.041 0.010 0.563
Replicate - 3 0.186 0.148 0.146 0.042 0.042 0.010 0.574
Mean 0.184 0.148 0.145 0.041 0.041 0.010 0.570
RSD 1.26 0.88 1.0 1.09 1.34 1.46 1.07
4.4.2) Conclusion:
1) Relative standard deviation for the mean values of assay of Withanoside IV in the samples is
<2.5% except WS/05Lot20 where it is 4.5% because the contents are very low.
2) Relative standard deviation for the mean values of assay of Withanoside V in sample is
<2.5% except WS/05Lot20 where it is 3.92% because the contents are very low.
3) Relative standard deviation for the mean values of assay of Withaferin A in sample is <2.5%
except WS/05Lot20 where it is 4.68% because the contents are very low.
4) Relative standard deviation for the mean values of assay of 12-Deoxy withastramonolide in
<2.5% except WS/05Lot20 where it is 5.13% because the contents are very low.
5) Relative standard deviation for the mean values of assay of Withanolide A in sample is
<2.5% except WS/05Lot20 where it is 5.46% because the contents are very low.
6) Relative standard deviation for the mean values of assay of Withanolide B in sample is
<2.5% except WS/05Lot20 where it is 3.68% because the contents are very low.
Enclosure-7: Standard chromatograms (3 Pages)
Enclosure-8: Sample chromatograms (24 pages).