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1$785$/ 5(0(',(6
3DJH RI
20
µ
l of standard solution dilution-3 (Refer 4.2.4) and sample solutions were injected and
chromatograms were recorded. From the obtained chromatograms
individual withanolides
content
were calculated.
b) Standard preparation for Spike recovery: Refer section 4.2.4 (Dilution-4)
Table-55: WS/05Lot21: Weight of sample: 453.70 mg - 100ml with methanol
Marker
Actual
content
(% w/w)
Weight
of
sample
(mg)
Actual
quantity
in the
weight
taken
Quantity
added
(mg)
Purity
Total
Quantity
(mg)
Calculated
Values
(%w/w)
Observed
Value
(%w/w)
Recovery
(%w/w)
W-IV
0.399
1.81
0.91
90
2.629
0.570
0.550
96.45
W-V
0.319
1.447
2.53
90
2.358
0.511
0.494
96.54
WF-A
0.413
1.874
1.03
99
2.893
0.627
0.622
99.15
12-D
0.123
0.558
1.09
95
1.594
0.346
0.324
93.69
W –A
0.107
0.485
1.03
99
1.505
0.326
0.306
93.72
W –B
0.030
453.7
0.136
0.90
99
1.027
0.223
0.237
106.48
Table-56: WS/06LoT10: Weight of sample: 328.90mg - 100ml with methanol
Marker
Actual
content
(% w/w)
Weight
of
sample
(mg)
Actual
quantity
in the
weight
taken
Quantity
added
(mg)
Purity
Total
Quantity
(mg)
Calculated
Values
(%w/w)
Observed
Value
(%w/w)
Recovery
(%w/w)
W-IV
0.804
2.684
1.08
90
3.656
1.087
1.028
94.62
W-V
0.684
2.256
2.38
90
3.113
0.925
0.901
97.40
WF-A
0.606
2.016
1.00
99
3.006
0.894
0.857
95.86
12-D
0.266
0.878
1.00
95
1.828
0.543
0.508
93.39
W –A
0.208
0.710
0.91
99
1.611
0.479
0.465
97.18
W –B
0.064
328.90
0.214
1.15
99
1.352
0.402
0.399
99.30
Table-57:
RD/1170
: Weight of sample: 313.9
mg
- 100ml with methanol
Marker
Actual
content
(% w/w)
Weight
of
sample
(mg)
Actual
quantity
in the
weight
taken
Quantity
added
(mg)
Purity
Total
Quantity
(mg)
Calculated
Values
(%w/w)
Observed
Value
(%w/w)
Recovery
(%w/w)
W-IV
0.746
2.342
0.94
90
3.188
0.992
0.951
95.88
W-V
0.644
2.022
2.43
90
2.896
0.901
0.864
95.82
WF-A
0.690
2.166
1.14
99
3.295
1.025
0.998
97.30
12-D
0.335
1.052
0.97
95
1.973
0.614
0.597
97.19
W –A
0.239
0.750
0.91
99
1.651
0.514
0.495
96.41
W –B
0.079
313.9
0.248
1.02
99
1.258
0.391
0.384
98.05