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1$785$/ 5(0(',(6
3DJH RI
Experiment-2 (Condition - 2)
b) Standard / sample preparation: Refer 4.3.11
Table-66:
Batch No.
Withanoside
IV
Withanoside
V
Withaferin
A
12-Deoxy
withastra
monolide
Withanolide
A
Withanolide
B
Sum
WS/06Lot08
1.071
0.629
0.608
0.304
0.290
0.071
2.974
WS/06Lot10
1.047
0.601
0.561
0.282
0.258
0.067
2.817
WS/05Lot20
0.080
0.044
0.083
0.024
0.027
0.0005
0.263
WS/05Lot21
0.538
0.298
0.415
0.130
0.128
0.027
1.537
RD/1170
0.995
0.599
0.740
0.257
0.348
0.080
3.020
RD/1045
0.744
0.357
0.422
0.140
0.201
0.043
1.906
RD/1162
0.162
0.083
0.090
0.022
0.025
0.0004
0.386
ERH-46
0.213
0.147
0.141
0.046
0.403
0.011
0.601
Table-67:
Batch No.
Condition
Sum
1
2.974
2
2.802
WS/06Lot08
RSD
2.977839
1
2.817
2
2.681
WS/06Lot10
RSD
2.473627
1
0.263
2
0.241
WS/05Lot20
RSD
4.365079
1
1.537
2
1.453
WS/05Lot21
RSD
2.809365
1
3.020
2
2.779
RD/1170
RSD
4.155889
1
1.906
2
1.793
RD/1045
RSD
3.05488
1
0.386
2
0.391
RD/1162
RSD
0.643501
1
0.601
2
0.570
ERH-46
RSD
2.64731
4.6.5) Conclusion:
Relative standard deviations for same batch sample when analysed in two
different instruments found to be < 6.0% indicating the method is very rugged.
Enclosure 12:
Standard and sample chromatograms
(27 pages)