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3DJH RI
The mean response factor for
Withanolide A
between the concentration 21.59 to 172.76 mcg/ml is
24803.79.
Table-72:
Different Concentrations
Withanolide A
(mcg/ml)
Dilution -6
(21.59)
Dilution -5
(43.19)
Dilution -4
(86.38)
Dilution -3
(172.76)
Dilution -2
(345.51)
Dilution -1
(691.02)
S. solution
(1382.04)
Mean
551256
1084444
2127011
4138065
7961571
13486375
13768071
Response factor
Area /
concentration
25527.76
25109.41
24624.59
23953.38
23042.95
19516.62
9962.14
Mean
(Dilution 3 to 6)
24803.79
±5.0% of 24803 is 23562 to 26043
The mean response factor for
Withanolide B
between the concentration 21.09 to 168.79 mcg/ml is
25634.15.
Table-73:
Different Concentrations
Withanolide B
(mcg/ml)
Dilution -6
(21.09)
Dilution -5
(42.19)
Dilution -4
(84.39)
Dilution -3
(168.79)
Dilution -2
(337.59)
Dilution -1
(675.18)
S. solution
(1350.36)
Mean
552858
1094892
2153855
4197521
7949510
12726145
12163116
Response factor
Area /
concentration
26202.60
25946.08
25520.36
24867.57
23547.83
18848.52
9007.31
Mean
(Dilution 3 to 6)
25634.15
±5.0% of 25634 is 24352 to 26915
Though the linearity is observed through out the six concentrations ranging from 20 to 600mcg.
The following limits are considered as a better working range based on linearity and closeness
(within ± 5%) to mean response factor.
Table-74:
Name of the analyte
Concentration (mcg/ml)
Withanoside IV
20.64 to 330.3
Withanoside V
18.76 to 300.15
Withaferin A
23.05 to 184.39
12-Deoxy withastramonolide
22.21 to 177.65
Withanolide A
21.59 to 172.76
Withanolide B
21.09 to 168.79