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3DJH RI
Table-33: Retention time of Withanoside V in sample (B. No. WS/06Lot10)
Replicate
Conc-1
(753.6 mg/100ml)
Conc-2
(1503.2 mg/100ml)
Conc-3
(2096.0 mg/100ml)
Replicate - 1
19.684
19.534
19.630
Replicate - 2
19.532
19.540
19.562
Mean
19.608
19.537
19.596
RSD
0.39
0.02
0.17
Table-34: Retention time of Withaferin A
Different Concentrations
Withaferin A
(mcg/ml)
Dilution -6
(23.05)
Dilution -5
(46.09)
Dilution -4
(92.19)
Dilution -3
(184.39)
Dilution -2
(368.78)
Dilution -1
(737.55)
S. solution
(1475.10)
Replicate – 1
20.32
20.328
20.374
20.383
20.337
20.563
20.337
Replicate – 2
20.311
20.332
20.351
20.353
20.349
20.347
20.344
Replicate – 3
20.327
20.341
20.362
20.355
20.314
20.344
20.352
Replicate – 4
20.306
20.339
20.345
20.357
20.361
20.342
20.352
Replicate – 5
20.333
20.336
20.349
20.355
20.525
20.335
20.352
Mean
20.319
20.335
20.356
20.361
20.377
20.386
20.347
RSD
0.05
0.03
0.06
0.06
0.41
0.49
0.03
Table-35: Retention time of Withaferin A in sample (B. No. WS/06Lot10)
Replicate
Conc-1
(753.6 mg/100ml)
Conc-2
(1503.2 mg/100ml)
Conc-3
(2096.0 mg/100ml)
Replicate - 1
20.362
20.224
20.308
Replicate - 2
20.223
20.227
20.248
Mean
20.293
20.226
20.278
RSD
0.48
0.01
0.15
Table-36: Retention time of 12-Deoxy Withastramonolide
Different Concentrations
12-Deoxy
withastramonolide
(mcg/ml)
Dilution -6
(22.21)
Dilution -5
(44.41)
Dilution -4
(88.83)
Dilution -3
(177.65)
Dilution -2
(355.30)
Dilution -1
(710.60)
S. solution
(1421.20)
Replicate – 1
21.351
21.351
21.391
21.394
21.36
21.542
21.36
Replicate – 2
21.341
21.358
21.367
21.367
21.366
21.366
21.365
Replicate – 3
21.357
21.361
21.38
21.376
21.341
21.363
21.373
Replicate – 4
21.338
21.362
21.365
21.367
21.382
21.362
21.37
Replicate – 5
21.356
21.358
21.37
21.376
21.51
21.353
21.372
Mean
21.349
21.358
21.375
21.376
21.392
21.397
21.368
RSD
0.04
0.02
0.05
0.05
0.32
0.38
0.03