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3DJH RI
Table-37: Retention time of 12-Deoxy Withastramonolide in sample (B. No. WS/06Lot10)
Replicate
Conc-1
(753.6 mg/100ml)
Conc-2
(1503.2 mg/100ml)
Conc-3
(2096.0 mg/100ml)
Replicate - 1
21.378
21.261
21.330
Replicate - 2
21.260
21.262
21.284
Mean
21.319
21.262
21.307
RSD
0.28
0.002
0.11
Table-38: Retention time of Withanolide A
Different Concentrations
Withanolide A
(mcg/ml)
Dilution -6
(21.59)
Dilution -5
(43.19)
Dilution -4
(86.38)
Dilution -3
(172.76)
Dilution -2
(345.51)
Dilution -1
(691.02)
S. solution
(1382.04)
Replicate – 1
22.219
22.216
22.255
22.253
22.225
22.391
22.219
Replicate – 2
22.209
22.226
22.23
22.23
22.225
22.227
22.224
Replicate – 3
22.224
22.225
22.245
22.241
22.206
22.221
22.229
Replicate – 4
22.206
22.229
22.231
22.227
22.247
22.223
22.224
Replicate – 5
22.221
22.224
22.235
22.241
22.364
22.213
22.228
Mean
22.216
22.224
22.239
22.062
22.253
22.255
22.225
RSD
0.04
0.02
0.05
0.05
0.29
0.34
0.02
Table-39: Retention time of Withanolide A in sample (B. No. WS/06Lot10)
Replicate
Conc-1
(753.6 mg/100ml)
Conc-2
(1503.2 mg/100ml)
Conc-3
(2096.0 mg/100ml)
Replicate - 1
22.234
22.126
22.192
Replicate - 2
22.126
22.128
22.149
Mean
22.180
22.127
22.171
RSD
0.24
0.005
0.097
PEAK AREA OF WITHANOLIDE B
Table-40: Retention time of Withanolide B
Different Concentrations
Withanolide B
(mcg/ml)
Dilution -6
(21.09)
Dilution -5
(42.19)
Dilution -4
(86.38)
Dilution -3
(172.76)
Dilution -2
(345.51)
Dilution -1
(675.18)
S. solution
(1350.36)
Replicate – 1
25.517
25.509
25.544
25.53
25.514
25.639
25.521
Replicate – 2
25.511
25.526
25.52
25.521
25.514
25.525
25.527
Replicate – 3
25.515
25.516
25.538
25.536
25.512
25.516
25.522
Replicate – 4
25.51
25.526
25.529
25.514
25.551
25.521
25.516
Replicate – 5
25.509
25.52
25.53
25.529
25.625
25.513
25.52
Mean
25.512
25.519
25.532
25.526
25.543
25.543
25.521
RSD
0.01
0.03
0.04
0.03
0.19
0.21
0.02