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3DJH RI
Table-41: Retention time of Withanolide B in sample (B. No. WS/06Lot10)
Replicate
Conc-1
(753.6 mg/100ml)
Conc-2
(1503.2 mg/100ml)
Conc-3
(2096.0 mg/100ml)
Replicate - 1
25.530
25.449
25.499
Replicate - 2
25.448
25.450
25.476
Mean
25.489
25.450
25.488
RSD
0.16
0.0020
0.06
4.3.10) Conclusion:
1)
Relative standard deviation for the mean values of retention times for Withanoside IV,
Withanoside V, Withaferin A, 12-Deoxy withastramonolide, Withanolide A and Withanolide
B in standard mix dilution is < 0.1%.
2)
Relative standard deviation for the mean values of retention times for Withanoside IV,
Withanoside V, Withaferin A, 12-Deoxy withastramonolide, Withanolide A and Withanolide
B in sample is < 0.1%.
For Standard chromatograms refer
Enclosure-5
Enclosure – 6:
Sample chromatograms
(6 Pages)