194 / 227
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3DJH RI
Table-19: Peak area of Withanoside IV in sample (B. No. WS/06Lot10)
Replicate
Conc-1
(753.6 mg/100ml)
Conc-2
(1503.2 mg/100ml)
Conc-3
(2096.0 mg/100ml)
Replicate – 1
478028
949957
1320439
Replicate - 2
479376
949797
1333648
Mean
478702
949877
1327044
RSD
0.14
0.01
0.50
Table-20: Peak area of Withanoside V
Different Concentrations
Withanoside
V
(mcg/ml)
Dilution -6
(18.76)
Dilution -5
(37.52)
Dilution -4
(75.04)
Dilution -3
(150.08)
Dilution -2
(300.15)
Dilution -1
(600.30)
S. solution
(1200.60)
Replicate – 1
151294
298008
586819
1146136
2238822
4334899
8731747
Replicate – 2
151121
297953
585559
1146843
2237319
4327804
8721728
Replicate – 3
151156
298036
585906
1141122
2243282
4330341
8719358
Replicate – 4
151277
297352
581818
1152990
2237337
4338646
8718285
Replicate – 5
150985
297517
584964
1151745
2248262
4345473
8714428
Mean
151167
297773
585013
1147767
2241004
4335433
8721109
RSD
0.08
0.11
0.33
0.42
0.21
0.16
0.07
Table-21: Peak area of Withanoside V in sample (B. No. WS/06Lot10)
Replicate
Conc-1
(753.6 mg/100ml)
Conc-2
(1503.2 mg/100ml)
Conc-3
(2096.0 mg/100ml)
Replicate - 1
401702
793298
1090118
Replicate - 2
394834
783540
1102054
Mean
398268
788419
1096086
RSD
0.86
0.62
0.54
Table-22: Peak area of Withaferin A
Different Concentrations
Withaferin A
(mcg/ml)
Dilution -6
(23.05)
Dilution -5
(46.09)
Dilution -4
(92.19)
Dilution -3
(184.39)
Dilution -2
(368.78)
Dilution -1
(737.55)
S. solution
(1475.10)
Replicate – 1
573810
1134850
2231492
4332623
8325259
14666731
21469032
Replicate – 2
573224
1134742
2227105
4337796
8322031
14756049
21438480
Replicate – 3
573669
1134589
2228288
4310236
8317825
14770374
21482780
Replicate – 4
573097
1134172
2211324
4335168
8328440
14788044
21438918
Replicate – 5
572942
1131663
2224755
4340882
8338317
14782253
21442185
Mean
573348
1134003
2224593
4331341
8326374
14752690
21454279
RSD
0.07
0.05
0.03
0.06
0.06
0.34
0.10