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3DJH RI
Dilution-6:
5ml of Dilution-5 solution was pipetted to10ml volumetric flask and made up to 10ml
with acetonitrile.
Table-10
Sl. No.
Reference standard
Concentrations (mcg/ml)
1.
Withanoside IV
20.64
2.
Withanoside V
18.76
3.
Withaferin A
23.05
4.
12-Deoxy withastramonolide
22.21
5.
Withanolide A
21.59
6.
Withanolide B
21.09
b)
Procedure:
All the above 1, 2, 3, 4, 5 and 6 dilutions were injected five times each and
chromatograms were recorded. A graph was plotted using concentrations on X-axis and
the respective peak areas on Y-axis. Correlation co-efficient
r
2
was calculated.
4.2.5) Observation:
Correlation co-efficient
r
2
for
Compound
r
2
Concentration range(mcg/ml)
Withanoside IV
0.999
20.0 to 1321
Withanoside V
0.999
18.7 to 1200
Withaferin A
0.996
23.0 to 737
12-Deoxy Withastramonolide
0.990
22.0 to 355
Withanolide A
0.992
21.0 to 345
Withanolide B
0.984
21.0 to 337