185 / 227
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3DJH RI
PEAK AREA OF WITHANOSIDE IV
Table-11
Different Concentrations
Withanoside IV
(mcg/ml)
Dilution -6
(20.64)
Dilution -5
(41.29)
Dilution -4
(82.58)
Dilution -3
(165.15)
Dilution -2
(330.30)
Dilution -1
(660.60)
S. solution
(1321.20)
Replicate – 1
166047
329878
651382
1275513
2493967
4815460
9597190
Replicate –2
166038
330019
651009
1278440
2493747
4811411
9587035
Replicate –3
166015
329943
651630
1269142
2491031
4819826
9584608
Replicate –4
166025
329827
649769
1278566
2492809
4815725
9588582
Replicate – 5
165993
329140
651869
1279224
2497552
4818317
9579369
Mean
166024
329761
651132
1276177
2493821
4816148
9587357
Response factor
Area /
concentration
8042.32
7986.95
7885.34
7727.38
7550.17
7290.57
7256.55
Mean
(Dilution 2 to 6)
7838.43
RSD
(Dilution 2 to 6)
2.29
LINEARITY GRAPH PEAK AREA Vs CONCENTRATION