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3DJH RI
Chromatogram – 3: Raw material chromatogram
When analysed using PDA detector the retention times of Withanoside IV, Withanoside V,
Withaferin A, 12-Deoxy withastramonolide, Withanolide A and Withanolide B were about
16.373, 20.565, 21.376, 22.368, 23.211 and 26.475 respectively (Refer chromatogram-
4,5,6,7,8,9,10 & 11). The PDA spectra of all the withanolides peaks in the sample are
identical to that of the respective standards. The purity of peaks due to all the withanolides
in the sample as well as standard are >0.98. The sample chromatogram has many other
peaks than six withanolides.
Chromatogram – 4: Standard Withanoside IV
Chromatogram – 5: Standard Withanoside V