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Natural Remedies Pvt Ltd.

Quality control Dept.

Sample ID : Withanolide B

Method Name

: C:\CLASS-VP\Methods\METHODS\Withania.met

Data Name

: D:\2007\Method validation\Withania somnifera\Jan 2007\160107\spl-06a

Injection Volume

: 20

Acquired

: 1/16/07 9:58:51 PM

Minutes

0

5

10

15

20

25

30

35

40

45

mAU

0

500

1000

1500

mAU

0

500

1000

1500

26.475

26.944 27.275

Detector

A-227 nm

Retention

Time

Area Area

Percent

Resolution Asymmetry Theoretica

l plates

Capacity

factor

Width USP

Width

Height

26.475 8139020 99.20

0.00

1.13

413752.75 0.53

0.49 0.16 138429

3

26.944

40938

0.50

2.81

1.23

407283.75 0.56

0.34 0.17 6620

27.275

25063

0.31

1.87

1.12

349493.94 0.58

0.33 0.18 3846

Totals

8205021 100.00

139475

9

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