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Natural Remedies Pvt Ltd.

Quality control Dept.

Sample ID : Withania somnifera : ERH - 46

Method Name

: C:\CLASS-VP\Methods\METHODS\Withania.met

Data Name

: D:\2007\Method validation\Withania somnifera\Jan 2007\160107\spl-13

Injection Volume

: 20

Acquired

: 1/17/07 4:19:17 AM

Minutes

0

5

10

15

20

25

30

35

40

45

mAU

0

50

100

150

200

250

mAU

0

50

100

150

200

250

16.576

20.661

21.440

22.389

23.243

26.496

Detector

A-227 nm

Retention

Time

Area Area

Percent

Resolution Asymmetry Theoretica

l plates

Capacity

factor

Width USP

Width

Height

16.576

537308 15.13

0.00

1.08

145898.22 -0.04

0.24 0.17 87496

20.661

453713 12.78

22.24

1.01

181706.16 0.20

0.29 0.19 65400

21.440 1723297 48.54

3.82

1.39

161359.55 0.24

0.48 0.21 211277

22.389

357061 10.06

5.00

1.02

291271.69 0.30

0.24 0.17 59849

23.243

380726 10.72

5.14

1.04

314259.59 0.35

0.26 0.17 64237

26.496

98366

2.77

20.08

1.23

449097.84 0.54

0.21 0.16 17761

Totals

3550471 100.00

506020

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