9 / 227
D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Std-rep005.lcd
min
mV
0
5
10
15
20
25
30
35
40
45
0
500
1000
1Det.A Ch1
Det.A Ch1/227nm
1
Detector A Ch1 227nm
Peak #
1
2
3
4
5
6
Total
Ret. Time
15.516
19.692
20.366
21.379
22.242
25.539
Name
Withanoside IV
Withanoside V
Withaferin A
12- Deoxy withastramonolide
Withanollide A
Withanolide B
Area
1234072
391438
5318748
5001196
3786871
2839005
18571330
Area %
6.645
2.108
28.640
26.930
20.391
15.287
100.000
Resolution
0.000
22.163
3.454
5.576
5.030
19.518
Acquired by
: Admin
Sample Name
: Withania : std mix
Sample ID
: 13.01.07
Vail #
: 40
Injection Volume
: 20 uL
Data File Name
: Std-rep005.lcd
Method File Name
: Withaina.met.lcm
Batch File Name
: Batch 01.lcb
Report File Name
: Calibration.lcr
Data Acquired
: 1/15/2007 7:15:23 AM
Data Processed
: 3/29/2007 4:24:59 PM
Chromatogram
D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Std-rep005.lcd
min
mV
15.0
17.5
20.0
22.5
25.0
0
500
1000
1Det.A Ch1
Det.A Ch1 / 227nm
1