11 / 227
D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II001.lcd
min
mV
0
5
10
15
20
25
30
35
40
45
0
100
200
1Det.A Ch1
Det.A Ch1/227nm
1
Detector A Ch1 227nm
Peak #
1
2
3
4
5
6
7
8
9
10
11
12
13
14
Total
Ret. Time
15.187
15.376
18.909
19.537
20.226
21.263
22.128
22.311
22.804
23.524
23.891
24.112
24.625
25.447
Name
RT15.187
Withanolside IV
RT18.909
Withanoside V
Withaferin A
Withastramonolide
Withanolide A
RT22.311
RT22.804
RT23.524
RT23.891
RT24.112
RT24.625
Withanolide B
Area
225004
478462
3921
400277
1097206
548663
398098
43911
36992
100800
15874
17872
6589
122702
3496369
Area %
6.435
13.685
0.112
11.448
31.381
15.692
11.386
1.256
1.058
2.883
0.454
0.511
0.188
3.509
100.000
Resolution
0.000
1.135
23.432
3.776
3.516
5.550
5.034
1.219
3.406
3.854
1.951
1.423
3.252
5.045
Acquired by
: Admin
Sample Name
: Withania somnifera : WS / 06 lot 10
Sample ID
: 750 mg / 100 ml :Trl 1
Vail #
: 43
Injection Volume
: 20 uL
Data File Name
: Rep-RP-II001.lcd
Method File Name
: Withaina.met.lcm
Batch File Name
: Batch 01.lcb
Report File Name
: Calibration.lcr
Data Acquired
: 1/16/2007 1:45:20 PM
Data Processed
: 3/29/2007 5:01:03 PM
Chromatogram
D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Rep-RP-II001.lcd
min
mV
15.0
17.5
20.0
22.5
25.0
0
100
200
1Det.A Ch1
Det.A Ch1 / 227nm
1