12 / 227
D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan008.lcd
min
mV
0
5
10
15
20
25
30
35
40
45
0
100
200
1Det.A Ch1
Det.A Ch1/227nm
1
Detector A Ch1 227nm
Peak #
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Total
Ret. Time
15.352
15.547
18.980
19.698
20.372
21.377
22.239
22.491
22.903
23.619
23.987
24.208
24.567
24.709
25.523
Name
RT15.352
Withanoside-IV
RT18.980
Withanoside V
Withaferin A
Withastramonolide
Withanolide A
RT22.491
RT22.903
RT23.619
RT23.987
RT24.208
RT24.567
RT24.709
Withanolide B
Area
265649
491157
8341
473414
1250895
410796
384409
1440
54369
36688
38306
14767
4931
4074
102080
3541316
Area %
7.501
13.869
0.236
13.368
35.323
11.600
10.855
0.041
1.535
1.036
1.082
0.417
0.139
0.115
2.883
100.000
Resolution
0.000
1.158
21.729
4.128
3.455
5.561
5.143
1.589
2.662
4.654
2.373
1.282
2.088
0.994
5.790
Acquired by
: Admin
Sample Name
: Withania somnifera ext
Sample ID
: RM - ERH - 46
Vail #
: 21
Injection Volume
: 20 uL
Data File Name
: Quan008.lcd
Method File Name
: Withaina.met.lcm
Batch File Name
: Batch 01.lcb
Report File Name
: Calibration.lcr
Data Acquired
: 1/15/2007 1:21:18 PM
Data Processed
: 3/29/2007 4:45:13 PM
Chromatogram
D:\LC2010AHT\2007\Method validation\Withania somnifera\Jan 2007\Quan008.lcd
min
mV
15.0
17.5
20.0
22.5
25.0
0
100
200
1Det.A Ch1
Det.A Ch1 / 227nm
1