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Table 4a: Independent Study - Matrices and Inoculating Organisms
1
Matrix
Inoculating
Organism
(Culture Condition)
Target Inoculum Level
# of
Replicates
MDA2
Testing Time
Points
Reference Method
(Enrichment)
Beef Hot Dogs
L. monocytogenes
ATCC
1
7644
(heat-stressed)
0 CFU/Test Portion
5
24 hours
USDA/FSIS MLG
8.09
(UVM)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Deli Turkey
L. monocytogenes
ATCC
1
19116
(heat-stressed)
0 CFU/Test Portion
5
24 hours
USDA/FSIS MLG
8.09
(UVM)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Queso Fresco
L. monocytogenes
CWD
2
1554
(heat-stressed)
0 CFU/Test Portion
5
24 hours
AOAC 993.12
(Selective
Enrichment)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Vanilla Ice
Cream
L. monocytogenes
ATCC
1
19114
0 CFU/Test Portion
5
24 hours
AOAC 993.12
(Selective
Enrichment)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
4% Milk Fat
Cottage Cheese
L. monocytogenes
ATCC
1
19112
&
L. welshimeri
ATCC
1
35897
0 CFU/Test Portion
5
24 hours
AOAC 993.12
(Selective
Enrichment)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Whole Melon
L. monocytogenes
FSL
3
J1-049
0 CFU/Test Portion
5
26 hours
FDA/BAM
Chapter 10
(BLEB)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Raw Chicken
Leg Pieces
Naturally
Contaminated
Lot 1
&
Lot 2
20
&
20
28 hours
USDA/FSIS MLG
8.09
(UVM)
Romaine
Lettuce
L. monocytogenes
ATCC
1
49594
0 CFU/ Test Portion
5
24 hours
FDA/BAM
Chapter 10
(BLEB)
0.2-2 CFU/ Test Portion
20
2-5 CFU/ Test Portion
5
3% Chocolate
Whole Milk
L. monocytogenes
ATCC
1
BAA-751
0 CFU/ Test Portion
5
24 hours
AOAC 993.12
(Selective
Enrichment)
0.2-2 CFU/ Test Portion
20
2-5 CFU/ Test Portion
5
Sealed Concrete
L. monocytogenes
ATCC1 19117
0 CFU/4” x 4” (100 cm2)
5
24 and 26
hours
USDA/FSIS MLG
8.09
(UVM)
~50 CFU/4” x 4” (100
cm2)
20
~100 CFU/4” x 4” (100
cm2)
5
Plastic
L. monocytogenes
ATCC1 51782&
L. seeligeri
ATCC1 35967&
10x Enterococcus
faecalis
ATCC1 29212
0 CFU/1” x 1” (5 cm2)
5
24 and 26
hours
USDA/FSIS MLG
8.09
(UVM)
~50 CFU/1” x 1” (5 cm2)
20
~100 CFU/1” x 1” (5
cm2)
5
2
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