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Table 7: Inoculum Summary Table for Whole Melons and Environmental Surfaces
Matrix Whole Melons
Inoculating Organism Listeria monocytogenes FSL J1-049
Low-Inoculum Level
CFUa/Melonb 7
High-Inoculum Level
CFUa/Test Melonb 55
Matrix Stainless Steel
Inoculating Organism Listeria monocytogenes
ATCC 19118
Enterococcus faecium
ATCC 19434
Low-Inoculum Level
CFU a/Test Areac 42 530
High-Inoculum Level
CFU a/Test Areac 400 4000
Matrix Sealed Concrete
Inoculating Organism Listeria monocytogenesATCC 19117
Low-Inoculum Level
CFU a/Test Areac 35
High-Inoculum Level
CFU a/Test Areac 360
Matrix Plastic
Inoculating Organism Listeria monocytogenes
ATCC 51782
Enterococcus faecalis
ATCC 29212
Low-Inoculum Level
CFUa /Test Aread 42 540
High-Inoculum Level
CFUa /Test Aread 420 3600
aCFU: aliquots of the inocula were plated in triplicate onto TSA and averaged
bTest Area: Whole Melon
cTest Area: 4” x 4” Surface Area
d Test Area: 1” x 1” Surface Area
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-30 D/ PTM Validation Report 081501
OMA ERP - June 2016
ERP Use Only