309 / 596
Table 4a: Independent Study - Matrices and Inoculating Organisms (Continued)
1
2
Matrix
Inoculating
Organism
(Culture Condition)
Target Inoculum Level
# of
Replicates
MDA2
Testing Time
Points
Reference Method
(Enrichment)
Stainless Steel
L. monocytogenes
ATCC 19118 &
10x Enterococcus
faecium
ATCC 19434
0 CFU/1” x 1” (5 cm2)
5
24 and 26
hours
USDA/FSIS MLG
8.09
(UVM)
~50 CFU/1” x 1” (5 cm2)
20
0 CFU/4” x 4” (100 cm2)
5
1
ATCC - American Type Culture Collection
3
2
CWD - University of Vermont
4
3
FSL - Cornell University
5
*
Natural contamination was present during the background screen: 2 separate lots evaluated (20 replicates each)
6
7
8
9
10
Table 4b: Internal Study - Matrices and Inoculating Organisms
11
Matrix
Inoculating
Organism
(Culture Condition)
Target Inoculum Level
# of
Replicates
MDA2
Testing Time
Points
Reference Method
(Enrichment)
Raw chicken
leg pieces
L. monocytogenes
Ad
1
668
0 CFU/Test Portion
5
28 hours
USDA/FSIS MLG
8.09
(UVM)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Bagged raw
spinach
L. monocytogenes
Ad 543
0 CFU/Test Portion
5
24 hours
ISO 11290-1/A1
(Half/Demi Fraser)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Cold smoked
salmon
L. monocytogenes
Ad 670
0 CFU/Test Portion
5
24 hours
FDA/BAM
Chapter 10
(BLEB)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
Cold smoked
salmon
L. monocytogenes
Ad 670
0 CFU/Test Portion
5
24 hours
ISO 11290-1/A1
(Half/Demi Fraser)
0.2-2 CFU/Test Portion
20
2-5 CFU/Test Portion
5
1
Ad – Adria Developpement Culture Collection
12
13
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-30 D/ PTM Validation Report 081501
OMA ERP - June 2016
ERP Use Only