477 / 596
5
and the s
R
provides the
variabilityvariance
in data between different laboratories. The P
T
value
1
provides information on the homogeneity test of laboratory PODs
.[10].
2
3
4
5
6
7
AOAC Official Method 2016.xxx
8
Listeria monocytogenes
in SelectFoods and Environmental Surfaces
9
3M
™
Molecular Detection Assay(MDA) 2-
Listeria monocytogenes
Method
10
First Action 2016
11
12
(Applicable to detection of
Listeria monocytogenes
hot dogs (25g & 125g), salmon (25g), deli
13
turkey (25g & 125g), cottage cheese (25g), chocolate milk (25 mL), vanilla ice cream (25g),
14
queso fresco (25g), romaine lettuce (25g), melon (whole), raw chicken leg pieces (25g); raw
15
chicken breast fillet (25g), concrete (3M™ Hydrated Sponge Stick with Dey-Engley [D/E] , 225
16
mL & 100 mL), stainless steel (3M Hydrated Sponge Stick with D/E, 225 mL), and plastic
17
(3M™ Enviro
sS
wab
with Letheen, 10 mL) environmental samples.
18
19
See Tables
2016.1A
and
2016.1B
for a summary of results of the inter-laboratory study.
20
See Tables
2016.2A
and
2016.2B
for detailed results of the inter-laboratory study
21
22
A.
Principle
23
24
The 3M
™
Molecular Detection Assay (MDA) 2 -
Listeria monocytogenes
method is used with
25
the 3M
™
Molecular Detection System (MDS) for the rapid and specific detection of
Listeria
26
monocytogenes
in enriched food and food process environmental samples. The 3M MDA2 -
27
Listeria monocytogenes
uses loop-mediated isothermal amplification of unique DNA target
28
sequences with high specificity and sensitivity, combined with bioluminescence to detect the
29
amplification. Presumptive positive results are reported in real-time while negative results are
30
displayed after the assay is completed. Samples arepre-enriched in Demi Fraser with ferric
31
ammonium citrate broth.
32
33
B.
Apparatus and Reagents
34
Items (b)-(g) are available as the 3M
™
Molecular Detection Assay (MDA) 2 -
Listeria
35
monocytogenes
kit from 3M Food Safety (St. Paul, MN 55144-1000, USA).
36
(a)
3M Molecular Detection System
(MDS100) – Available from 3M Food Safety (St.
37
Paul, MN 55144-1000, USA).
38
(b)
3M Molecular Detection Assay 2 – Listeria monocytogenes reagent tubes-
12 strips of
39
8 tubes. Available from 3M Food Safety (St. Paul, MN 55144-1000, USA).
40
(c)
Lysis Solution (LS) tubes –
12 strips of 8 tubes.
41
(d)
Extra caps
– 12 strips of 8 caps
42
(e)
Reagent Control –
8 reagent tubes
43
(f)
Quick Start Guide
44
(g)
3M
™
Molecular Detection Speed Loader Tray -
Available from 3M Food Safety
45
(St. Paul, MN 55144-1000, USA).
46
(h)
3M
™
Molecular Detection Chill Block Insert
- Available from 3M
™
Food Safety (St.
47
Paul, MN 55144-1000, USA).
48
(i)
3M
™
Molecular Detection Heat Block Insert
- Available from 3M Food Safety
49
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