478 / 596
6
(St. Paul, MN 55144-1000, USA).
1
(j)
3M
™
Molecular Detection Cap/Decap Tool for Reagent tubes
- Available from 3M
2
Food Safety (St. Paul, MN 55144-1000, USA).
3
(k)
3M
™
Molecular Detection Cap/Decap Tool for Lysis tubes
– Available from 3M
4
Food Safety (St. Paul, MN 55144-1000, USA).
5
(l)
Empty Lysis Tube Rack
- Available from 3M Food Safety (St. Paul, MN 55144-1000,
6
USA).
7
(m)
Empty Reagent Tube Rack
- Available from 3M Food Safety (St. Paul, MN 55144-
8
1000, USA).
9
(n)
Demi Fraser Broth -
Available from 3M Food Safety (St. Paul, MN 55144-1000,
10
USA).
11
(o)
Ferric Ammonium Citrate (FAC),
ACS grade, 5% sterilized
-
Available from MP
12
Biomedicals™ or equivalent.
13
(p)
Disposable pipette
– capable of 20 µL
14
(q)
Multi
-
channel (8-channel) pipette
- capable of 20 µL
15
(r)
Sterile filter tip pipette tips
- capable of 20 µL
16
(s)
Filter Stomacher® bags –
Seward or equivalent
.
17
(t)
Stomacher
®– Seward or equivalent.
18
(u)
Thermometer –
calibrated range to include 100 ± 1
o
C
19
(v)
Dry block heater unit–
capable of maintaining 100 ± 1
o
C
20
(w)
Incubators.
– Capable of maintaining 37 ± 1°C
or 41.5 ± 1°C.
21
(x)
Freezer –
capable of maintaining -10 to -20
o
C, for storing the 3M Molecular
22
Detection Chill Block Tray
23
(y)(x)
Refrigerator –
capable of maintaining 2-8
o
C, for storing the 3M Molecular
24
Detection Assay components
25
(z)(y)
Computer –
compatible with the 3M
™
Molecular Detection Instrument
26
(aa)(z)
3M™ Enviro
s S
wab, --
(hydrated with Letheen) Available from 3M Food
27
Safety(Australia)
28
(bb)(aa)
3M™ Hydrated Sponge Stick with 10 mL of D/E –
Available from 3M
29
Food Safety(St. Paul, MN 55144-1000, USA)
30
31
C. General Instructions
32
33
(a)
Store the 3M Molecular Detection Assay 2 -
Listeria monocytogenes
at 2-8°C. Do not
34
freeze. Keep kit away from light during storage. After opening the kit, check that the
35
foil pouch is undamaged. If the pouch is damaged, do not use. After opening, unused
36
reagent tubes should always be stored in the re-sealable pouch with the desiccant
37
inside to maintain stability of the lyophilized reagents. Store resealed pouches at 2-
38
8°C for no longer than 60 days. Do not use 3M Molecular Detection Assay 2 -
Listeria
39
monocytogenes
past the expiration date.
40
(b)
Follow all instructions carefully. Failure to do so may lead to inaccurate results.
41
Safety Precautions
42
The 3M MDA2 –
Listeria monocytogenes
is intended for use in a laboratory
43
environment by professionals trained in laboratory techniques. 3M has not
44
documented the use of this product in industries other than the food and beverage
45