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industries. For example, 3M has not documented this product for testing drinking
1
water, pharmaceutical, cosmetics, clinical or veterinary samples. The 3M MDA2 –
2
Listeria monocytogenes
has not been evaluated with all possible food products, food
3
processes, testing protocols or with all possible strains of bacteria.
4
As with all test methods, the source of enrichment medium can influence the results.
5
The 3M MDA 2 –
Listeria monocytogenes
has only been evaluated for use with the
6
enrichment media specified in the Instructions for Use section.
7
The 3M™ Molecular Detection instrument is intended for use with samples that have
8
undergone heat treatment during the assay lysis step, which is designed to destroy
9
organisms present in the sample. Samples that have not been properly heat treated
10
during the assay lysis step may be considered a potential biohazard and should NOT
11
be inserted into the 3M MDS instrument.
12
The user should read, understand and follow all safety information in the instructions
13
for the 3M MDS and the 3M MDA 2 –
Listeria monocytogenes
. Retain the safety
14
instructions for future reference.
15
To reduce the risks associated with exposure to chemicals and biohazards:
16
Performpathogen testing in a properly equipped laboratory under the control of
17
trained personnel.Always follow standard laboratory safety practices, including
18
wearing appropriate protective apparel and eye protection while handling reagents
19
and contaminated samples.Avoid contact with the contents of the enrichment media
20
and reagent tubes after amplification.Dispose of enriched samples according to
21
current industry standards.
22
Listeria monocytogenes
is of particular concern for pregnant women, the aged and the
23
infirmed. It is recommended that these concerned groups avoid handling this
24
organism.After use, the enrichment medium and the 3M MDA 2 -
Listeria
25
monocytogenes
tubes can potentially contain pathogenic materials.Periodically
26
decontaminate laboratory benches and equipment (pipettes, cap/decap tools, etc.) with
27
a 1- 5% (v:v in water) household bleach solution or DNA removal solution. When
28
testing is complete, follow current industry standards for the disposal of contaminated
29
waste. Consult the Safety Data Sheet for additional information and local regulations
30
for disposal.
31
To reduce the risks associated with environmental contamination:Follow current
32
industry standards for disposal of contaminated waste.
33
D.
Sample Enrichment
34
35
Foods
36
(a)
Allow the Demi-Fraser Broth enrichment medium (includes ferric ammonium citrate)
37
to equilibrate to ambient laboratory temperature (20-25
o
C).
38
(b)
Aseptically combine the enrichment medium and sample according to Table
2A
. For
39