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Transfer each sample lysate into individual Reagent tubes first followed by the NC. Hydrate
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the RC tube last.
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5.
Use the 3M™ Molecular Detection Cap/Decap Tool-Reagent to decap the Reagent tubes –
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one Reagent tube strip at a time. Discard cap.
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5.1
Transfer 20 µL of Sample lysate from the upper ½ of the liquid (avoid precipitate) in the
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LS tube into corresponding Reagent tube. Dispense at an angle to avoid disturbing the
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pellets. Mix by gently pipetting up and down 5 times.
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5.2
Repeat step 5.1 until individual Sample lysate has been added to a corresponding
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Reagent tube in the strip.
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5.3
Cover the Reagent tubes with the provided extra caps and use the rounded side of the
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3M Molecular Detection Cap/Decap Tool-Reagent to apply pressure in a back and
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forth motion ensuring that the cap is tightly applied.
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5.4
Repeat step 5.1 as needed, for the number of samples to be tested.
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5.5
When all sample lysates have been transferred, repeat 4.1 to transfer 20 µL of NC
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lysate into a Reagent tube.
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5.6
Transfer 20 µL of NC lysate into a RC tube. Dispense at an angle to avoid disturbing
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the pellets. Mix by gently pipetting up and down 5 times.
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6.
Load capped tubes into a clean and decontaminated 3M Molecular Detection Speed Loader
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Tray. Close and latch the 3M Molecular Detection Speed Loader Tray lid.
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7.
Review and confirm the configured run in the 3M Molecular Detection Software.
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8.
Click the Start button in the software and select instrument for use. The selected
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instrument’s lid automatically opens.
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9.
Place the 3M Molecular Detection Speed Loader Tray into the 3M Molecular Detection
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Instrument and close the lid to start the assay. Results are provided within 75 minutes,
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although positives may be detected sooner.
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AOAC Research Ins itute
Expert Review Panel Use Only
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol
OMA ERP - June 2016
ERP Use Only