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1
R
EFERENCES
:
2
1.
US Food and Drug Administration Bacteriological Analysis Manual. Chapter 10: Detection
3
and Enumeration of
Listeria monocytogenes
in Foods. Section C-6. April 2011 Version.
4
2.
US Department of Agriculture (USDA) FSIS Microbiology Laboratory Guidebook 8.08.
5
Isolation and Identification of
Listeria monocytogenes
from Red Meat, Poultry and Egg
6
Products, and Environmental Samples. Effective Date: 6 Nov 2012.
7
3.
ISO 11290-1. Microbiology of Food and Animal Feeding Stuffs – Horizontal Method for the
8
Detection and Enumeration of
Listeria monocytogenes
. Amendment 1, 2004-10-15.
9
4.
ISO/IEC 17025. General requirements for the competence of testing and calibration
10
laboratories.
11
5.
ISO 7218. Microbiology of food and animal feeding stuffs – General rules for microbiological
12
examination.
13
6.
ISO 18593. Microbiology of food and animal feeding stuffs – Horizontal methods for
14
sampling techniques from surfaces using contact plates and swabs.
15
16
Appendix A.
Protocol Interruption: Storage and re-testing of heat-treated lysates
17
1.
To store a heat-treated lysate, re-cap the lysis tube with a clean cap (see “L
YSIS
”, 4.5)
18
2.
Store at 4 to 8°C for up to 72 hours.
19
3.
Prepare a stored sample for amplification by inverting 2-3 times to mix.
20
4.
Decap the tubes.
21
5.
Place the mixed lysate tubes on 3M Molecular Detection Heat Block Insert and heat at 100
22
±1°C for 5 ±1 minutes.
23
6.
Remove the rack of LS tubes from the heating block and allow to cool in the 3M Molecular
24
Detection Chill Block Insert at least 5 minutes and a maximum of 10 minutes.
25
7.
Continue the protocol at the ‘Amplification’ section detailed above.
26
27
EXPLANATION OF PRODUCT LABEL SYMBOLS
28
29
Caution or Warning, see product instructions
30
31
Consult product instructions
32
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol
OMA ERP - June 2016
ERP Use Only