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1
2
P
REPARATION OF THE
3M™M
OLECULAR
D
ETECTION
C
HILL
B
LOCK
I
NSERT
3
Place the 3M™ Molecular Detection Chill Block directly on the laboratory bench; (the 3M™
4
Molecular Detection Chill Block Tray is not used). Use the chill block at ambient laboratory
5
temperature (20-25°C).
6
7
P
REPARATION OF THE
3M™M
OLECULAR
D
ETECTION
H
EAT
B
LOCK
I
NSERT
8
Place the 3M™ Molecular Detection Heat Block Insert in a dry double block heater unit. Turn on
9
the dry block heater unit and set the temperature to allow the 3M Molecular Detection Heat
10
Block Insert to reach and maintain a temperature of 100 ±1°C.
11
12
NOTE: Depending on the heater unit, allow approximately 30 minutes for the 3M Molecular
13
Detection Heat Block Insert to reach temperature. Using an appropriate, calibrated thermometer
14
(e.g., a partial immersion thermometer or digital thermocouple thermometer, not a total
15
immersion thermometer) placed in the designated location, verify that the 3M Molecular
16
Detection Heat Block Insert is at 100 ±1°C.
17
18
P
REPARATION OF THE
3M™M
OLECULAR
D
ETECTION
I
NSTRUMENT
19
1.
Launch the 3M™ Molecular Detection Software and log in.
20
2.
Turn on the 3M Molecular Detection Instrument.
21
3.
Create or edit a run with data for each sample. Refer to the 3M Molecular Detection System
22
User Manual for details.
23
24
NOTE: The 3M Molecular Detection Instrument must reach and maintain temperature of 60°C
25
before inserting the 3M Molecular Detection Speed Loader Tray with reaction tubes. This
26
heating step takes approximately 20 min and is indicated by an ORANGE light on the
27
instrument’s status bar. When the instrument is ready to start a run, the status bar will turn
28
GREEN.
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30
L
YSIS
31
1.
Allow the lysis solution (LS) tubes to warm up by setting the rack at room temperature (20-
32
25 °C) overnight (16-18 hours). Alternatives to equilibrate the LS tubes to room
33
AOAC Research Institute
Expert Review Panel Use Only
OMAMAN-29/OMAMAN-30 Combined Collaborative Study Protocol
OMA ERP - June 2016
ERP Use Only