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3M MDA2 E. coli O157:H7 Collaborative Study
May 2016
OMA-2016-May-XXXX
DRAFT DOCUMENT
6
e)
Most Probable Number (MPN) Analysis.
The level of
E. coli
O157 will be determined by MPN
1
on the day of analysis by evaluating 5 x 650 g, 20 x 325 g (using the reference method test
2
portions) or 5 x 325 g and 5 x 160 g inoculated test samples. Each test portion will be analyzed
3
by the reference method procedure. The number of positives from the 3 test levels was used to
4
calculate the MPN using the LCF MPN calculator available at the AOAC RI website.
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6
3)
3M™ Molecular Detection Assay 2 -
E. coli
O157 Method
7
8
a)
Applicability
9
10
For the detection of
E. coli
O157
from: raw ground beef (325 g), frozen blueberries (25 g),
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sprouts (25 g), fresh baby spinach (200 g).
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13
b)
Test Kit Information
14
a)
Kit Name.—
3M™ Molecular Detection Assay 2 –
E. coli
O157 (including H7)
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b)
Catalog Number
.—
MDA2ECO96
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17
c)
Test Kit Reagents -
3M MDA2 -
E. coli O157
(including H7) (96 tests)
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a)
Lysis Solution (LS) tubes
. – 96 (12 strips of 8 tubes)
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b)
E. coli O157 Reagent tubes
. – 96 (12 strips of 8 tubes)
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c)
Extra caps.
– 96 (12 strips of 8 caps)
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d)
Reagent Control (RC).
– 16 (2 pouches of 8)
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e)
Quick Start Guide
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d)
Additional Supplies and Reagents
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a)
3M Molecular Detection System (instrument), power supply, cord, USB cable and software
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b)
3M Molecular Detection Speed Loader Tray
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c)
3M Molecular Detection Chill Block Tray and Chill block insert
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d)
3M Molecular Detection Heat Block Insert
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e)
3M Molecular Detection Cap/Decap Tool [Reagent]
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f)
3M Molecular Detection Cap/Decap Tool [Lysis]
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g)
Empty lysis tube rack
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h)
Empty reagent tube rack
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i)
3M™ Enrichment Pouch with 225mL of Buffered Peptone Water (ISO formulation) and filter,
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available from 3M #QEBPWI225F
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j)
Matrix Control, available from 3M #MDMC96NA
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37
e)
Additional Media for Confirmation
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a)
Modified Rainbow agar (mRBA)
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b)
Tryptic soy agar with 5% sheep blood (SBA)
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c)
Dynal antibody coated paramagnetic beads
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d)
Biochemical confirmation supplies
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e)
Serological confirmation supplies
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f)
Apparatus
45
a)
Pipettes
. – Capable of 20µL
46
b)
Multi-channel pipette
. – Capable of 20µL
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c)
Sterile pipette tips.
– Capable of 20µL
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OMAMAN-35 B: Collaborative Study Protocol
ERP USE ONLY
January 2017
AOAC Research Institute
Expert Review Panel Use Only