AOAC-RI ERP Book MICRO.pdf

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4.0 DISCUSSION
The purpose of this study was to validate the 3M TM MDA for detection of E. coli
O157:H7 in frozen blueberries. This study followed the format for the validation of
a candidate method using the experimental setup and Probability of Detection
(POD) statistical analysis provided in the February 24, 2012 version of the AOAC
International Methods Committee Guidelines for Validation of Microbiological
Methods for Food and Environmental Surfaces document (1).
Overall, based on the evidence presented here, the 3MTM MDA is a valid method
for the detection of E. coli O157:H7 in frozen blueberries. The POD statistical
analysis of the results for the candidate method and the reference method
identified that these methods are not significantly different from each other.
Additionally, no false positives or false negatives were identified by comparing the
‘screening stage’ and the ‘confirmation stage’ of the candidate method. This
validation is accurate for the target sample size (25 g) tested.
5.0REFERENCES
1. Brunelle S, LaBudde R, Nelson M, Wehling P. 2012. AOAC International
Methods Committee Guidelines for Validation of Microbiological Methods for
Food and Environ-mental Surfaces. Available at:
_Validation_Guidelines_for_Food_Microbiology-Prepub_version.pdf.
Accessed: 11/30/15.
2. Feng P, Weagant SD, Jinneman, K. 2011. U.S. Food and Drug
Administration Bacteriological Analytical Manual, Chapter 4A: Diarrheagenic
Escherichia coli. Available at:
80.htm. Accessed: 11/30/15.
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