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CONFIDENTIAL INFORMATION
Page 12 of 13
Overall, based on the results of this study, the
3M™
Molecular Detection Assay
(MDA) is a valid method for the detection of
Escherichia coli
O157:H7 in 325 g
ground beef (18 hr enrichment). The candidate methods explored here with the
targeted sample sizes produce
equivalent results in both the ‘screening stage’ and
the ‘confirmation stage’ and produce equivalent results when compared with the
USDA MLG reference method, typically employed for ground beef.
5.0 REFERENCES
1.
Brunelle S, LaBudde R, Nelson M, Wehling P
. 2012. AOAC International
Methods Committee Guidelines for Validation of Microbiological Methods for
Food
and
Environ-mental
Surfaces.
Available
at:
http://www.aoac.org/imis15_prod/AOAC_Docs/StandardsDevelopment/AOAC_Validation_Guidelines_for_Food_Microbiology-Prepub_version.pdf.
Accessed: 11/30/15.
2.
United States Department of Agriculture, Food Safety Inspection
Service, Office of Public Health Science
. 2014. Microbiology Laboratory
Guidebook Method 5.09: Detection, Isolation and Identification of
Escherichia
coli
O157:H7 from Meat Product and Carcass and Environmental Sponges.
Available at:
http://www.fsis.usda.gov/wps/wcm/connect/51507fdb-dded-47f7-862d-ad80c3ee1738/MLG-5pdf?MOD=AJPERES. Accessed: 11/30/2015.
6.0 DATA DISPOSITION
Copies of raw data and final reports from this study will be archived at Food Safety
Net Services, Ltd., 199 W. Rhapsody, San Antonio, TX 78216, and shall be kept
c
onfidential as per the Agreement between FSNS and 3M™.