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QIAGEN
mericon
STEC Workflow Collaborative Study Protocol
May 2016
DRAFT
22
QIAGEN
mericon
® STEC Workflow Collaborative Study
Materials provided by collaborator
Apparatus (standard lab apparatus):
(a)
Incubator
.-Capable of maintaining 42 ± 1°C.
(b)
Top-loading balance.-
Capable of weighing 1-2000 g.
(c)
Laboratory paddle blender.-
Seward 3500 or equivalent.
(d)
Vortex mixer.-
Multiple laboratory suppliers (MLS).
(e)
Microcentrifuge.-
For 1.5-2.0 mL microcentrifuge tubes.(minimum of 100 required for MLG
confirmation)
(f)
Pipettors.-
Adjustable, capable of delivering 10-100 μL and 100-1000 μL.
(g)
Dry bath incubator.-
Capable of maintaining 100 ± 1°C.
(h)
Calibrated thermometer.-
Capable of measuring 100 ± 1°C.
(i)
Miltenyi Biotech Octomacs magnets or equivalent (required for MLG confirmation)
(j)
Labquake agitator or equivalent (required for MLG confirmation)
Consumables:
(a)
Serological pipettes
– Aerosol resistant
(b)
Micropipette tips
, aerosol resistant.-MLS.
(c)
Microtubes
, 2.0 mL, with caps.-MLS.
(d)
Water.
-Nuclease-free PCR grade, or equivalent, MLS.
(e)
Sterile Petri Dishes
.-100 x 15 mm, MLS.
Materials provided by QIAGEN
Kits supplied by QIAGEN:
(a)
mericon®
E. coliO157 Screen Plus
Pathogen Detection Assay
(24),
CAT# 290403
(b)
mericon®
E. coliSTEC O-Type
Pathogen Detection Assay
(24),
CAT# 290233
(c)
mericon®
DNA Bacteria Kit (100), CAT#69525
(d)
QIAsymphony
®
mericon®
DNA Bacteria Kit, CAT# 931156
Apparatus and accessories supplied by QIAGEN:
(a)
Thermocycler
.-RotorGene Q®, QIAGEN #9001640, with notebook computer containing Rotor-Gene
Q® software version 2.3.1.49 or later.
(b)
QIAsymphony® SP.-
QIAGEN #9001297, for automated DNA extraction.
(c)
QIAsymphony® AS.-
QIAGEN #9001301, for automated PCR assay setup.
(d)
Rotor.-
QIAGEN #9018903, 72 wells, for holding 0.1 mL strip tubes and caps.
(e)
Locking ring for 72 well rotor
.-QIAGEN #9018904, for locking strip tubes and caps in 72-well rotor.
OMAMAN-36 B : Collaborative Study Protocol
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only