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QIAGEN
mericon
STEC Workflow Collaborative Study Protocol
May 2016
DRAFT
23
(f)
Rotor-Disc 72 Rotor.-
QIAGEN #9018899.
(g)
Locking ring for Rotor-Disc 72.-
QIAGEN #9018900.
(h)
Reagent cartridge holder (2).-
QIAGEN #997008.
(i)
Sample carrier insert, 2 mL v2
.-QIAGEN #9242083, secondary tube adapter for 2 mL screw-cap
tubes.
(j)
Cooling adapter.-
QIAsymphony EMT v2, QIAGEN #920730.
(k)
Reagent holder cooling adapter.-
QIAGEN #9018090.
(l)
Adapter.-
2 x Rotor-Disc, for use with QIAsymphony, QIAGEN #9242204.
(m) Rotor-Disc 72 loading block.-
QIAGEN #9018910. Store refrigerated to ensure that PCR setup is
performed under stable thermal conditions.
(n)
Rotor-Disc heat sealer
.-QIAGEN #9018898 (110 V) or #9019725 (230 V).
Consumables supplied by QIAGEN:
(a)
Rotor-Disc 72
QIAGEN #981301 (24) or # 981303 (240)
(b)
Rotor-Disc heat sealing film
.-QIAGEN #981601 (60) or #981604 (600).
(c)
Sample preparation cartridges
.-8-well, QIAGEN #997002.
(d)
8-Rod covers
.-QIAGEN #997004.
(e)
Elution microtubes.-
CL with cap strips, QIAGEN #19588.
(f)
Filter tips.-
1500 μL, QIAGEN #997024.
(g)
Filter tips.-
200 μL, QIAGEN #990332.
(h)
Filter tips.-
50 μL, QIAGEN #997120.
Materials provided by QLabs:
(a) Sample sets packaged in filter stomacher bags
(b) Dehydrated enrichment media (mTSB + CAA)
(c) Prepared IMS reagents (E-Buffer)
(d) Large Cell Separation Column
(e) 50 mL Conical Vials
(f) 0.45 µm Cell Strainers
(g) Rainbow Agar
(h) Cefiximine
(i) Potassium Tellurite Solution
(j) Novobiocin Solution
(k) DuPont Nutrition and Health BAX Systems STEC PCR Screening Assay
(l) Remel O157 Latex Agglutination Test Kit
(m) VITEK 2 GN or API 20 E Biochemical Identification Kits
(n) Prepared Sheep Blood Agar
Methods provided by QLabs
OMAMAN-36 B : Collaborative Study Protocol
For ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only