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mericon
E. coli Detection Workflows 8/2015
Contents
Kit Contents 5 Storage and Shelf Life 7 Intended Use 7 Intended User 7 Applicability 8 Environmental Factors 8 Safety Information 9 Technical Assistance 9 Principle of the assays 10 General Precautions for Real-Time PCR Assays 12 Equipment and Reagents to Be Supplied by User 12 Automated Workflow 12 Manual Workflow 14 Real-Time PCR 15 Specifications of the AOAC-RI PTM-Certified E. coli O157 Screen Plus and E. coli STEC O-type Detection Workflow 16 Confirmation of Positive Results: 18 Protocol: Preparation of E. coli O157 Screen Plus and E. coli STEC O-type Enrichment Cultures – Food Matrices 19 Automated Workflow 19 Protocol: Automated isolation of bacterial DNA on the QIAsymphony SP 19 Protocol: Assay setup on the QIAsymphony AS 21 Manual Workflow 23 Protocol: Manual isolation of DNA using the mericon DNA Bacteria Kit 23 Protocol: Manual assay setup 24 Real-Time PCR 25 Protocol: PCR and data analysis on the Rotor-Gene Q using the open mode software 25 Data Analysis on the Rotor-Gene Q 29OMAMAN-36 C: Method User Guide/Package Insert
ERP Use Only
January 2017
AOACResearch Institute
ExpertReview Panel Use Only