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mericon
E. coli Detection Workflows 8/2015
11
Table1. Targets and channels of the mericon E. coli O157 Screen Plus Assay
Green
channel:
FAM™
(495/520
nm)
Orange
channel:
ROX™
NHS Ester
(588/608
nm)
Crimson
channel:
Cy
®
5.5
(685/707
nm)
Yellow
channel:
MAX™
NHS Ester
(524/557
nm)
mericon
E. coli O157
Screen Plus
Assay
stx1
/
stx2
O157
eae
Internal
control (IC)
Table 2. Targets and channels of the mericon E. coli STEC O-Type Assay
Green Channel
FAM
™
(495/520 nm)
Orange Channel
ROX
™
NHS Ester
(588/608 nm)
Yellow Channel
MAX
™
NHS
Ester (524/557
nm)
mericon
E. coli
STEC O-Type
Assay
O26, O45, O103,
O111, O121, O145
O157:H7
Internal Control
(IC)
Data Analysis
The Rotor-Gene Q cycler produces raw data files that are further interpreted by
the software using mathematical algorithms to characterize samples. The
software guides the user through each step and provides simplicity for
beginners as well as an experimental platform for advanced users. [2]
OMAMAN-36 C: Method User Guide/Package Insert
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only