AOAC-RI ERP Book MICRO.pdf

mericon

E. coli Detection Workflows 8/2015

Table 4. Validated matrix portions and culture volumes

Sample amount

Culture volume

Matrix

Ground Beef

325 g

975 ml

Raw Beef Trim

325 g

975 ml

Spinach

25 g

225 ml

Table 5. Limit of detection

Workflow segment

Limit of detection*

Overall automated and manual workflow

cfu/ml

mericon

E. coli O157 Screen Plus and

mericon

E. coli STEC O-type kit

10 copies/reaction

Limit of Detection is derived from method development studies

Table 6. Sample volumes for mericon assay setup

Manual workflow

eluate dilution

Automated workflow

elution volume

Matrix

Ground Beef

1:10

400 µl

Raw Beef Trim

1:10

400 µl

Spinach

1:10

400 µl

OMAMAN-36 C: Method User Guide/Package Insert

ERP Use Only

January 2017

AOAC Research Institute

Expert Revi w Panel Use Only