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Table 12:
mericon
E. coliSTEC O-Type PathogenDetection Assay for Raw Beef
Trim
*
Paired test portions were used in accordance with the approved study protocol
Raw Beef Trim (325 g)
E. coli
O26 ATCC BAA-1653
Low Level
0.89 (0.52, 1.51) MPN/Test Portion
Sample #
manual
mericon
QIASymphony
mericon
®
Confirmation
USDA/FSIS-
MLG 5B.05*
Time Point
Time Point
Time Point
10 Hour
10 Hour
10 Hour
1
+
+
+
+
2
+
+
+
+
3
-
-
-
-
4
-
-
-
-
5
-
-
-
-
6
+
+
+
+
7
+
+
+
+
8
+
+
+
+
9
+
+
+
+
10
+
+
+
+
11
-
-
-
-
12
+
+
+
+
13
-
-
-
-
14
+
+
+
+
15
-
+
-
-
16
+
+
+
+
17
-
-
-
-
18
+
+
+
+
19
+
+
+
+
20
+
+
+
+
Total
13/20
13/20
13/20
13/20
High Level
2.58 (1.15, 5.78) MPN/Test Portion
1
+
+
+
+
2
+
+
+
+
3
+
+
+
+
4
+
+
+
+
5
+
+
+
+
Total
5/5
5/5
5/5
5/5
Uninoculated
1
-
-
-
-
2
-
-
-
-
3
-
-
-
-
4
-
-
-
-
5
-
-
-
-
Total
0/5
0/5
0/5
0/5
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only