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Table 10:
mericon
E. coliSTEC O-Type PathogenDetection Assay for Raw Ground
Beef (70% Lean)
*
Paired test portions were used in accordance with the approved study protocol
Raw Ground Beef (70% Lean) (325 g)
E. coli
O157:H7 ATCC 43895
Low Level
0.75 (0.44, 1.23) MPN/Test Portion
Sample #
manual
mericon
QIASymphony
mericon
®
Confirmation
USDA/FSIS-
MLG 5.09*
Time Point
Time Point
Time Point
10 Hour
10 Hour
10 Hour
1
+
+
+
+
2
-
-
-
-
3
+
+
+
+
4
-
-
-
-
5
-
-
-
-
6
-
-
-
-
7
+
+
+
+
8
-
-
-
-
9
-
-
-
-
10
+
+
+
+
11
-
-
-
-
12
+
+
+
+
13
+
+
+
+
14
-
-
-
-
15
+
+
+
+
16
+
+
+
+
17
-
-
-
-
18
+
+
+
+
19
-
-
-
-
20
-
-
-
-
Total
9/20
9/20
9/20
9/20
High Level
3.70 (1.52, 9.02) MPN/Test Portion
1
+
+
+
+
2
+
+
+
+
3
+
+
+
+
4
+
+
+
+
5
+
+
+
+
Total
5/5
5/5
5/5
5/5
Uninoculated
1
-
-
-
-
2
-
-
-
-
3
-
-
-
-
4
-
-
-
-
5
-
-
-
-
Total
0/5
0/5
0/5
0/5
OMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only