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Table 6C: MPN Summary Table for Raw Beef Trim
Raw Beef Trim (325 g)
E. coli
O26 ATCC BAA-1653
Low Level Inoculum (0.2-2 MPN/Test Portion)
A
B
C
D
E
650 g
-
+
+
-
+
20 x 325 g (Reference Samples)
13/20
130 g
-
+
+
-
-
MPN/Test portion
0.89
Low Conf. Limit MPN/Test Portion
0.52
High Conf. Limit MPN/Test Portion
1.51
High Level Inoculum (2-5 MPN/Test Portion)
A
B
C
D
E
5 x 325 g (Reference Samples)
5/5
130 g
+
+
+
+
+
65 g
-
+
-
+
+
MPN/Test portion
2.58
Low Conf. Limit MPN/Test Portion
1.15
High Conf. Limit MPN/Test Portion
5.78
*
MPN was calculated for the low level inoculation for raw beef trim using five 650 g, twenty 325 g (reference
method test portions), and five 130 g using the LCF MPN Calculator version 1.6 provided by AOAC-RI
http://www.lcfltd.com/customer/LCFMPNCalculator*
MPN was calculated for the high level inoculation for raw beef trim using five 325 g (reference method test
portions), five 130 g and the five 65 g using the LCF MPN Calculator version 1.6 provided by AOAC-RI
http://www.lcfltd.com/customer/LCFMPNCalculatorOMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only