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Table 6B: MPN Summary Table for Raw Ground Beef (70% Lean)
Raw Ground Beef (70% Lean) (325 g)
E. coli
O157:H7 ATCC 43895
Low Level Inoculum (0.2-2 MPN/Test Portion)
A
B
C
D
E
650 g
+
+
+
-
+
20 x 325 g (Reference Samples)
9/20
130 g
-
+
-
+
+
MPN/Test portion
0.75
Low Conf. Limit MPN/Test Portion
0.44
High Conf. Limit MPN/Test Portion
1.23
High Level Inoculum (2-5 MPN/Test Portion)
A
B
C
D
E
5 x 325 g (Reference Samples)
5/5
130 g
+
+
+
+
+
65 g
-
+
+
+
+
MPN/Test portion
3.70
Low Conf. Limit MPN/Test Portion
1.52
High Conf. Limit MPN/Test Portion
9.02
*
MPN was calculated for the low level inoculation for raw ground beef using five 650 g, twenty 325 g
(reference method test portions), and five 130 g using the LCF MPN Calculator version 1.6 provided by
AOAC-R
I http://www.lcfltd.com/customer/LCFMPNCalculator*
MPN was calculated for the high level inoculation for raw ground beef using five 325 g (reference method
test portions), five 130 g and the five 65 g using the LCF MPN Calculator version 1.6 provided by AOAC-RI
http://www.lcfltd.com/customer/LCFMPNCalculatorOMAMAN-36 E/ AOAC PTM Report
ERP Use Only
January 2017
AOAC Research Institute
Expert Review Panel Use Only