372 / 696
4/6
Table 1. Precision and Spike recovery results for beta-carotene
Beta-carotene
Performance
Analytical range
10 μg/kg – 2.8 mg/kg
Limit of quantitation (LOQ)
≤ 10 μg/kg
Linearity (10 points)
0.01 – 10 mg/L: r
2
= 0.9993
Repeatability
(n = 6-fold, RSD
r
)
9.2 μg/kg (SM)
4.5 %
110 μg/kg (SM + spike)
0.9 %
520 μg/kg (IF)
4.2 %
2820 μg/kg (IF + spike)
2.9 %
Reproducibility
(n = 6 duplicates, RSD
R
)
9.2 μg/kg (SM)
110 μg/kg (SM + spike)
520 μg/kg (IF)
2820 μg/kg (IF + spike)
Recovery
(n = 6)
110 μg/kg (SM + spike)
97 %
2820 μg/kg (IF + spike)
109 %
SM = skimmed milk (liquid). IF = infant formula (powder).
Results are based on the total of
cis
and
trans
isomers.
Table 2. Precision and Spike recovery results for lutein
Lutein
Performance
Analytical range
71 μg/kg – 4.6 mg/kg
Limit of quantitation (LOQ)
≤ 71 μg/kg
Linearity (10 points)
0.02 – 3.3 mg/L: r
2
= 0.9999
Repeatability
(n = 6-fold, RSD
r
)
not detectable
(SM)
-
65 μg/kg (SM + spike)
2.4 %
1210 μg/kg (IF)
2.1 %
5020 μg/kg (IF + spike)
2.4 %
Reproducibility
(n = 6 duplicates, RSD
R
)
not detectable
(SM)
65 μg/kg (SM + spike)
1210 μg/kg (IF)
5020 μg/kg (IF + spike)
Recovery
(n = 6)
65 μg/kg (SM + spike)
98 %
5020 μg/kg (IF + spike)
101 %
SM = skimmed milk (liquid). IF = infant formula (powder).
Results are based on the total of
cis
and
trans
isomers.
Carot-01 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE