381 / 696
(g)
Calculate the contents of all-
trans
-β-carotene and
cis
isomers of β-carotene in the test samples:
BC
trans
= RF
BC
x (M
A
/M
S
) x (A
BC
/A
A
) x 100
Where BC
trans
= the all-
trans
-β-carotene in the sample (μg/100 g)
RF
BC
= the response factor for all-
trans
-β-carotene
M
A
= the mass of apocarotenal added to the test sample, in μg
M
S
= the sample weight, in g
A
BC
= the peak area of all-
trans
-β-carotene in the sample chromatogram (AU)
A
A
= the peak area of apocarotenal in the sample chromatogram (AU)
BC
cis
= RF
BC
x (M
A
/M
S
) x ([(A
15cisBC
x 1.4) + (A
13cisBC
x 1.2) + A
9cisBC
+ A
XcisBC
]/A
A
) x 100
Where BC
cis
= the
cis
isomers of β-carotene in the sample (μg/100 g)
RF
BC
= the response factor for all-
trans
-β-carotene
M
A
= the mass of apocarotenal added to the test sample, in μg
M
S
= the sample weight, in g
A
BC
= the peak area of all-
trans
-β-carotene in the sample chromatogram (AU)
A
15cisBC
= the peak area of 15-
cis
-β-carotene in the sample chromatogram (AU)
A
13cisBC
= the peak area of 13-
cis
-β-carotene in the sample chromatogram (AU)
A
9cisBC
= the peak area of 9-
cis
-β-carotene in the sample chromatogram (AU)
A
XcisBC
= the peak area of unidentified
cis
isomers of β-carotene in the sample
chromatogram (AU)
A
A
= the peak area of apocarotenal in the sample chromatogram (AU)
Carot-02 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE