385 / 696
Table 1. Calibration error for each carotenoid.
all-
trans
-Lutein
all-
trans
-β-Carotene
Apocarotenal
Conc
(μg/100 mL)
Residual
Conc
(μg/100 mL)
Residual
Conc
(μg/100 mL)
Residual
185.6
0.427848006
244.0
-1.561049661
257.0
1.663495109
92.8
1.169099574
122.0
4.765184349
128.5
-1.823403562
37.1
-4.622149486
48.8
-3.505075245
51.4
-3.373542764
18.6
1.855899172
24.4
-1.581828442
25.7
-1.846922498
3.71
-4.881101079
4.88
1.882768999
5.14
5.380373715
C.
Accuracy – Accuracy was determined by spike and recovery from SRM 1849a. SRM 1849a is not
fortified with lutein or β-carotene, but contains very low levels of β-carotene.
Table 2. Recovery of lutein and β-carotene spiked into SRM 1849a (n=3). Background levels of β-
carotene were taken into account.
all-
trans
-Lutein all-
trans
-β-Carotene
Spike level (μg/100 g as fed) 12.9
1.29
15.3
1.53
Avg recovery
99.0% 100.2%
101.9%
96.5%
%RSD
0.05% 0.18%
0.51%
0.62%
D.
Precision
Table 3. Repeatability of milk-based infant formula from SPIFAN sample set (n=3).
cis
-Lutein all-
trans
-Lutein
cis
-β-Carotene all-
trans
-β-Carotene
Avg (μg/100 g as fed)
ND
4.5
6.7
13.6
%RSD
n/a
0.27%
0.68%
0.29%
Table 4. Repeatability of PN soy-based infant formula (n=3).
cis
-Lutein all-
trans
-Lutein
cis
-β-Carotene all-
trans
-β-Carotene
Avg (μg/100 g as fed)
ND
4.1
7.3
15.2
%RSD
n/a
0.58%
1.04%
0.21%
E.
LOQ – Based on spike and recovery data a LOQ of 1.3 μg/100 g was observed for lutein and 1.5
μg/100 g for β-carotene.
F.
References
(a)
Aman et al. (2005).
Food Chem
. 92: 753-763.
(b)
Craft and Soares (1992).
J. Agric. Food Chem
. 40: 431-434.
(c)
Granado et al. (2001).
J. Food Comp. Anal
. 14: 479-489.
(d)
Marx et al. (2000).
Food Chem
. 70: 403-408.
(e)
Müller et al. (2008).
J. AOAC Int
. 91(6): 1284-1297.
(f)
Puspitasari-Nienaber et al. (2002).
JAOCS
79(7): 633-640.
(g)
Szpylka and DeVries (2005).
J. AOAC Int
. 88(5): 1279-1291.
(h)
The United States Pharmacopeial Convention (2014). Revision Bulletin - Beta Carotene.
(i)
Waysek et al. (2010).
J. AOAC Int
. 93(2): 499-509.
Carot-02 (February 2016)
FOR ERP USE ONLY
DO NOT DISTRIBUTE