390 / 696
B. Apparatus
24
Common laboratory equipment and, in particular, the following:
25
(a)
Analytical Balances
.-precision 0.01 mg and 0.1 mg.
26
(b)
Centrifuge
.-Tabletop with rotor for 50 mL conical tubes,
capable of operating at ≥ 12’000 g.
27
(c)
Centrifuge tubes
.-50 mL, conical, polypropylene.
28
(d)
Pipettes
.-1, 10, 20, 50 and 100 mL, Class A glass volumetric or automatic (Eppendorf or
29
equivalent).
30
(e)
One-mark
volumetric flasks
.-50, 100, 500, and 1000 mL glass, Class A.
31
(f)
Graduated cylinders.-
25 mL, 100 mL and 500 mL, glass.
32
(g)
Autosampler Beaker e.g. 120 mL, depending on the titrator used.
33
(h)
pH-meter / mV-meter, with a scale covering ± 700 mV, and burette, 20 mL or 25 mL,
34
Mettler-Toledo (Columbus, OH or equivalent).
35
(i)
Automatic titrator
(autosampler, motorized piston burette, with remote-control dispensing and
36
filling
).
(
Mettler T50, Roundo Tower autosampler, MettlerLabX 3.1 software or Metrohm 862
37
Compact Titrosampler, 800 Dosino, 10mL Exchange Unit, or equivalent). Alternatively a semi-
38
automated (e.g. MetrohmTitrado 905/907, with MetrohmTiamo
TM
software or equivalent) or
39
manual titrator (using a burette with accuracy of 0.01 mL) may be used.
40
(j)
Combined ring silver electrode e.g. Mettler DM 141 or DMi145-SC, Metrohm Ag Titrode
41
6.0430.100 or equivalent, alternatively a silver electrode with reference electrode.
42
(k)
Magnetic stirrer (Heidolph MR 3000, or Metrohm 804Ti Stand with 802 Rod Stirrer, or
43
equivalent).
44
(l)
Water bath (Labotech DWM 16 or equivalent).
45
(m
)
Ultrasonic cleaner (Tianjin Auto Science AS2060B or equivalent).
46
(n)
Disposable syringe. 3 mL, with handspike and 0.45
μ
m disposable syringe filter.
47
48
C. Chemicals and Reagents
49
(a)
Water, purified.
—
Greater than 18MΩ (EMD Millipore Corp., Billerica, MA, USA, or
50
equivalent).
51
(b)
Sodium chloride (NaCl)
,
(≥ 99.5%)
. C
ertified reference material for titrimetry, certified by
52
BAM, according to ISO 17025
—
Sigma Aldrich #71387 or equivalent.
53
(c
)
Silver Nitrate (
AgNO
3)
.
—
meets analytical specification of Ph. Eur., BP, USP, assay 99.8 -
54
100.5 %, Sigma-Aldrich 10220, or equivalent.
55
(d)
Potassium ferrocyanide trihydrate
(K
4
Fe(CN)
6
· 3H
2
O).
—
puriss. p.a., ACS reagent, reag.
56
ISO, reag. Ph. Eur., ≥99%, Sigma
-Aldrich # 31524 or
equivalent
.
57
2015.07/2015.08 (February 2016)
Chlor-02/Chlor-04
FOR ERP USE ONLY
DO NOT DISTRIBUTE